The Nextera DNA Flex Library Prep Kit provides library preparation reagent for DNA sequencing on Next Generation Sequencing system of Illumina.

Category:

Nextera DNA Flex Library Preparation Kit integrates the DNA extraction, fragmentation, library preparation, and library normalization steps to deliver the fastest, most flexible workflows in the Illumina library prep portfolio:

– Human Whole Genome Sequencing: applied in Cancer Genomics Research, Variant Detection, Genetic Risk Studies and Population Genetics. For example, sequencing Human Genome (3.2 Gb) on NovaSeq (8 samples/flow cell), HiSeq (8 samples/flow cell);

– Large, Complex Genomes Sequencing: applied in Agrigenomics (maize, wheat, bovine, etc.), Plant/Animal Research and Model Organisms (fruit fly, mouse, zebrafish, etc.). For example, gene sequencing of fruit fly (175 Mb) on NextSeq 550 (22 samples/flow cell), mouse (2.7 Gb) on HiSeq 4000 (8 samples/flow cell);

– Small Genomes Sequencing: applied in Human Microbiome, Microbiology/Metagenomics, Public Health Research, Amplicon Sequencing. For example, gene sequencing of E. Coli (4.6 Mb) on MiniSeq (50 samples/flow cell), plasmid/amplicons (650 kb) on MiSeq (11 samples/flow cell).

Parameters
Assay Time ~ 3.5-4 hours
Hands-on Time 1-1.5 hours
Specialised Sample Types gDNA, Blood, Saliva, PCR Amplicons, Plasmids, Dried Blood Spots, Low Input Samples
DNA Input Type ·   Small Genomes: 1-500 ng DNA,

·   Large Genomes: 100-500 ng

Technology Human Whole Genome, Small Whole Genome, Large Whole Genome of any species including Human Genome
Samples/run Maximum 96 samples
Insert Size 300-350 bp
Variant Class Chromosomal Abnormalities, Copy Number Variants (CNVs), Gene Fusions, Germline Variants, Insertions-Deletions (indels), Loss of Heterozygosity (LOH), Single Nucleotide Polymorphisms (SNPs), Somatic Variants, Structural Variants
NGS System Compatibility HiSeq 2500HiSeq 3000HiSeq 4000HiSeq X FiveHiSeq X TenMiniSeqMiSeqMiSeqDx in Research ModeNextSeq 550NovaSeq 6000
Compatitable Accessory Kit Nextera Index kit (24 hoặc 96 indexes)

 

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