Nextera DNA Flex Library Preparation Kit integrates the DNA extraction, fragmentation, library preparation, and library normalization steps to deliver the fastest, most flexible workflows in the Illumina library prep portfolio:
– Human Whole Genome Sequencing: applied in Cancer Genomics Research, Variant Detection, Genetic Risk Studies and Population Genetics. For example, sequencing Human Genome (3.2 Gb) on NovaSeq (8 samples/flow cell), HiSeq (8 samples/flow cell);
– Large, Complex Genomes Sequencing: applied in Agrigenomics (maize, wheat, bovine, etc.), Plant/Animal Research and Model Organisms (fruit fly, mouse, zebrafish, etc.). For example, gene sequencing of fruit fly (175 Mb) on NextSeq 550 (22 samples/flow cell), mouse (2.7 Gb) on HiSeq 4000 (8 samples/flow cell);
– Small Genomes Sequencing: applied in Human Microbiome, Microbiology/Metagenomics, Public Health Research, Amplicon Sequencing. For example, gene sequencing of E. Coli (4.6 Mb) on MiniSeq (50 samples/flow cell), plasmid/amplicons (650 kb) on MiSeq (11 samples/flow cell).