– Large Whole-Genome Sequencing (human, plant, animal)
– Small Whole-Genome Sequencing (microbe, virus)
– Exome Sequencing
– Targeted Gene Sequencing (amplicon, gene panel)
– RNA sequencing
– Methylation sequencing
– Shotgun Metagenomics
|
|
Flow Cell Type |
|
|
SP |
S1 |
S2 |
S4 |
Sequencing Output |
2 × 50 bp |
65–80 Gb |
134–167 Gb |
333–417 Gb |
N/A |
2 × 100 bp |
N/A |
266–333 Gb |
667–833 Gb |
1600–2000 Gb |
2 × 150 bp |
200–250 Gb |
400–500 Gb |
1000–1250 Gb |
2400–3000 Gb |
Quality Scores |
2 × 50 bp |
≥ 85% |
2 × 100 bp |
≥ 80% |
2 × 150 bp |
≥ 75% |
Run Time |
2 × 50 bp |
~13 hr |
~13 hr |
~16 hr |
N/A |
2 × 100 bp |
N/A |
~19 hr |
~25 hr |
~36 hr |
2 × 150 bp |
~25 hr |
~25 hr |
~36 hr |
~44 hr |
Details: https://sapac.illumina.com/systems/sequencing-platforms/novaseq.html