Model: MiSeq
Manufacturer: Illumina/USA
– The MiSeq System combines cluster generation, amplification, sequencing and data analysis on a single system
– The system is most widely used, with next-generation sequencing technology –  Sequencing by Synthesis – SBS

– Small Whole-genome resequencing
– Targeted sequencing
– HLA sequencing
– mRNA sequencing, small RNA sequencing
– Denovo sequencing
– 16S RNA sequencing in metagenomic analysis, identify pathogenic bacteria
– Sequencing to detect gene mutations in cancer research and targeted treatment
– Gene cloning checking
– Applied in parental determination, chromosome aneuploidy such as preimplantation genetic screening (PGS), forensic sciences

MiSeq System features:

– Maximum Output: 7.5-8.5 Gb within 39 hours (paired-end sequencing of 250 bp) when using MiSeq Reagent Kit v2; 13.2-15 Gb within 56 hours (paired-end sequencing of 300 bp) when using MiSeq Reagent Kit v3
– Data quality: Quality Score > Q30 (99.9% accuracy)
– Ability to paired-end sequencing of 2×300 bp.

Power of the MiSeq system:
– Sequencing time when using the MiSeq Reagent Kit Kit v2:

  • 1 x 36 bp ≤ 4 hours with the output of 540-610 Mb
  • 2 x 25 bp ≤ 5.5 hours with the output of 750-850 Mb
  • 2 x 150 bp ≤ 24 hours with the output of 4.5-5.1 Gb
  • 2 x 250 bp ≤ 39 hours with the output of 7.5-8.5 Gb

– Sequencing time when using the MiSeq Reagent Kit Kit v3:

  • 1 x 75 bp ≤ 21 hours with the output of 3.3-3.8 Gb
  • 2 x 300 bp ≤ 56 hours with the output of 13.2-15 Gb

See more at: https://sapac.illumina.com/systems/sequencing-platforms/miseq.html

 

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