Model: HiSeq 4000
Manufacturer: Illumina/USA
– Next-Generation Sequencing System uses sequencing technology: Sequencing by Synthesis – SBS
– The whole process of reading and sequencing is carried out on the surface of flow cells, convenient and easy handing
– The system is installed and operated maximum with 2 flow cells, the system can operate with each flow cell or simultaneously 2 flow cells depending the number of samples

Category:

– The system can sequence maximum of 12 human genomes with the coverage 30X, 96 exomes, or 100 transcriptomes in fewer than 3.5 days
– Maximum Output: 1.5 TB for paired-end sequencing of 2×250 bp within only 3.5 days
– Data output with 1 or 2 flow cells:

  • Maximum output of 1500 Gb for 2 x 150 bp sequencing
  • Maximum output of 750 Gb for 2 x 75 bp sequencing
  • Maximum output of 250 Gb for 1 x 50 bp sequencing

– Maximum clusters passing filter for 1 or 2 flow cells: 4.3-5 billion
– Sequencing on 1 or 2 flow cells:

  • Single read: 1 x 50 bp
  • Paired end: 2 x 75 bp
  • Paired end: 2 x 150 bp

– Data quality (Quality Score) > Q30 for 1 or 2 flow cells:

  • ≥ 75% of bases above Q30 for 2 x 150 bp sequencing
  • ≥ 80% of bases above Q30 for 2 x 75 bp sequencing
  • ≥ 85% of bases above Q30 for 2 x 50 bp sequencing

See more at: https://sapac.illumina.com/systems/sequencing-platforms/hiseq-3000-4000.html

– Single read (SR) or paired-end (PE) sequencing
– Exome sequencing
– Transcriptome sequencing (mRNA-Seq)
– The whole human genome sequencing
– Other sequencing methods

Additional information

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