The ZR DNA & ZR-96 DNA Sequencing Clean-Up Kits™ provide simple and rapid (2 & 10 minute) methods for removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data, including dye peaks or “dye blobs” which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide.
DNA Sequencing Clean-Up
Reusable and simple purification kit for preparing sequence-ready DNA:
– Complete elimination of “Dye Blobs” for high quality Phred scores and long read lengths.
– Flexible 6-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.
Elution Volume: ≥ 6 µl.
Sample Storage: Eluted DNA can be used immediately or stored at ≤ -20ºC.
Yield: Up to 5 µg DNA.
Sample Source: Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension products.